TABLE OF CONTENTS
Title page iCertification iiDedication iiiAcknowledgment ivTable of contents vAbstract viCHAPTER ONE1.1 Introduction 11.2 Justification 41.3 Aims and Objectives 4CHAPTER TWO2.1 Literature Review 52.2 Sperm Mophology 62.3 Sperm Quality 72.4 Sperm Motility 72.5 Membrane Integrity 82.6 Extenders 92.7 Cryoprotectants 112.8 Effect of cryopreservation on sperm motility and fertility 122.9 Computer Assisted Sperm Analysis of velocity 14
CHAPTER THREE3.0 Materials and Methods 173.1 Description of the study area 173.2 Collection and Management of fish 173.3 Methodology 183.3.1 Preparation of buffer 183.3.2 Preparation of Extender 183.3.3 Precurement and dilution of milt 193.3.4 Equilibration and Storage 203.3.5 Determination of Post-thawed parameters 21
CHAPTER FOUR4.1 Morphemetric Characteristics of Experimental Fish 224.2 Sperm Characteristics 234.3 Sperm Motility/mortality 244.4 % Mean Motility 25CHAPTER FIVE5.1 Discussion 285.2 Summary and Conclusion 29References 30Appendix 35
To service the growing demand of insufficient supply of (Clarias gariepinus) brookstock for aquaculture in Nigeria, Conserve valuable genetic resources and with the intent of obtaining a semen preservation protocol that can serve as a means of making fingerlings available to fish farmers all year round, this study assessed cryopreservation (Clarias gariepinus) of three specimen were discested and milt preserved. Result showed that there was significance difference between fresh semen and control. Cryopreservation of sperm, remains essentially a research activity that is just beginning to have commercial application. It is hope their efforts will be geared towards standardization of research result to enhance beneficial repeatability by interested fish farmers
1.2 JUSTIFICATIONAs fish farming expands and harvesting of wild stocks becomes more intense, there is a growing need for techniques of gamete storage to facilitate replacement of extinct fishes.Cryopreservation of sperm is now a widely used method for long term storage of fish milt. It is effective tool for conserving genetic materials in threatened and endangered species, which can also be used for genetic improvement through selecting breeding programme.
1.3 AIMS AND OBJECTIVE 1. To evaluate the sperm characteristics of C. gariepinus.2. To determine efficacy of some extenders in the preservation of milt of C. gariepinus.