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ASPECTS OF CRYOPRESERVATION OF Clarias gariepinusOBTAINED FROM THE LOWER RIVER NIGER

  • Department: AGRICULTURAL EXTENSION
  • Chapters: 1-5
  • Pages: 42
  • Attributes: Questionnaire, Data Analysis, Abstract
  • Views: 186
  • PRICE: ₦ 3,000
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TABLE OF CONTENTS

Title page                                                                                          iCertification                                                                                       iiDedication                                                                                         iiiAcknowledgment                                                                              ivTable of contents                                                                              vAbstract                                                                                            viCHAPTER ONE1.1     Introduction                                                                              11.2     Justification                                                                              41.3     Aims and Objectives                                                               4CHAPTER TWO2.1     Literature Review                                                                    52.2     Sperm Mophology                                                                   62.3     Sperm Quality                                                                         72.4     Sperm Motility                                                                        72.5     Membrane Integrity                                                                 82.6     Extenders                                                                                92.7     Cryoprotectants                                                                      112.8     Effect of cryopreservation on sperm motility and fertility         122.9     Computer Assisted Sperm Analysis of velocity                      14

CHAPTER THREE3.0     Materials and Methods                                                   173.1     Description of the study area                                         173.2     Collection and Management of fish                                173.3     Methodology                                                                  183.3.1  Preparation of buffer                                                    183.3.2  Preparation of Extender                                               183.3.3  Precurement and dilution of milt                                    193.3.4  Equilibration and Storage                                              203.3.5  Determination of Post-thawed parameters                    21

CHAPTER FOUR4.1     Morphemetric Characteristics of Experimental Fish        224.2     Sperm Characteristics                                                    234.3     Sperm Motility/mortality                                                   244.4     % Mean Motility                                                             25CHAPTER FIVE5.1     Discussion                                                                    285.2     Summary and Conclusion                                             29References                                                                             30Appendix                                                                                 35

ABSTRACT

To service the growing demand of insufficient supply of (Clarias gariepinus) brookstock for aquaculture in Nigeria, Conserve valuable genetic resources and with the intent of obtaining a semen preservation protocol that can serve as a means of making fingerlings available to fish farmers all year round, this study assessed cryopreservation (Clarias gariepinus) of three specimen were discested and milt preserved. Result showed that there was significance difference between fresh semen and control. Cryopreservation of sperm, remains essentially a research activity that is just beginning to have commercial application. It is hope their efforts will be geared towards standardization of research result to enhance beneficial repeatability by interested fish farmers

CHAPTER ONE

1.1 INTRODUCTION

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1.2     JUSTIFICATIONAs fish farming expands and harvesting of wild stocks becomes more intense, there is a growing need for techniques of gamete storage to facilitate replacement of extinct fishes.Cryopreservation of sperm is now a widely used method for long term storage of fish milt. It is effective tool for conserving genetic materials in threatened and endangered species, which can also be used for genetic improvement through selecting breeding programme.

1.3     AIMS AND OBJECTIVE 1.       To evaluate the sperm characteristics of C. gariepinus.2.       To determine efficacy of some extenders in the preservation of milt of C. gariepinus.

CHAPTER TWO

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